Fundamental and Practical Aspects in the Formulation of Colloidal Polyelectrolyte Complexes of Chitosan and siRNA

The formation of electrostatic interactions between polyanionic siRNA and polycations gives an easy access to the colloidal particles capable delivering in vitro or vivo. Among used for delivery, chitosan occupies a special place due its unique physicochemical biological properties. In this chapter we describe fundamental practical aspects complexes siRNA. basis complexation oppositely charged polyelectrolytes is first introduced with focus on specific conditions obtain stable colloid complex particles. Subsequent, properties that make so are described. third part, main parameters influencing stability siRNA/chitosan reviewed emphasis some consider preparation complexes.